Choose a tissue dissociator when the required endpoint is a viable single-cell suspension or gently separated tissue units.
Tissue type, enzyme conditions, mechanical action, temperature and processing time must be evaluated together; a grinder or homogenizer is not an automatic substitute when cell viability matters.
Use an exact released tissue dissociator and compatible vessel
Model requiredSterility, volume and mechanical compatibility must be
Compatibility requiredSelected by tissue and downstream workflow; record supplier, lot and concentration
Method dependentRecord digestion and handling temperature throughout the workflow
Controlled fieldFlow cytometry, culture, sequencing or other acceptance method
Workflow dependentCompare the route and compatible configuration before selecting a model for your sample.
Record tissue source, condition, amount, target cell population and the required yield and viability.
Select a reviewed enzyme system and vessel, then document sterility and temperature requirements.
Run the controlled mechanical and enzymatic sequence while recording program, time, temperature and observations.
Measure cell yield, viability, aggregates, debris and target-cell recovery before accepting or adjusting the method.
Intensity and cycle structure must be validated against viability and yield.
Record enzyme identity, concentration, lot and incubation conditions.
Excessive treatment can reduce viability or change surface markers.
Record both dissociation and interim handling temperatures.
Publish results only with the measurement method and tissue context.
Exact values depend on the sample, model, vessels and desired result. Use a sample test when available evidence does not match your workflow.
Rated equipment information.
Test conditions and results.
Results for the exact sample and method.
Model-specific application evidence.
